Reza Nassiri Rad; Mohammad Hossein Hadad Khodaparast; Amir Hossein Elhami Rad; Shiva Roufigari Haghighat
Abstract
Green Tea is prepared from the leaves of Camellia sinensis. Catechins of Green tea are well known as natural antioxidants and have effective beneficial on the body. One of the most important processes in the extraction of catechins and producing green tea beverage is brewing process. The object of this ...
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Green Tea is prepared from the leaves of Camellia sinensis. Catechins of Green tea are well known as natural antioxidants and have effective beneficial on the body. One of the most important processes in the extraction of catechins and producing green tea beverage is brewing process. The object of this study is to investigating the effect of brewing temperatures (60٫70 and 80˚c) and duration (5٫10 and 15 min) also the season effect of plucking on green tea that was produced in the Tea Research foundation of lahijan. results of total phenolic content on brewed green tea, is showed that plucking in summer and spring seasons samples have much total phenolic content are rather than plucking in autumn season samples also percent extraction of brewing green tea at 15 minuets has most highest total phenolic content than other seasons and brewing times .brewing temperature are not significant effected on the total phenolic content.
Seyedeh Mahsa Dadpour; Morteza Khomeiri; Hamidreza Sadeghi Pour; Shiva Rofigari Haghighat; Mehran Alami
Abstract
Fermentation is an important process for black tea production. During this process, by exposure of substrate to enzymes and the underlying oxidation reactions, quality determining factors of tea is produced. It is known that the exogenous application of cell-wall degrading enzymes leads to the increased ...
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Fermentation is an important process for black tea production. During this process, by exposure of substrate to enzymes and the underlying oxidation reactions, quality determining factors of tea is produced. It is known that the exogenous application of cell-wall degrading enzymes leads to the increased production of these compounds through enhancement of enzyme-substrate interactions. Pectinases in an important enzyme in this regard which is produced by microorganisms like genera of Aspergillus. In the present study, pectin lyase was isolated and partially purified through three- phase partitioning method from Aspergillus niger. The enzyme was purified by 16.65- fold from Aspergillus niger. The yield of purification was 52%. To assay the effects of purified enzymes on some qualitative characteristics of tea, Crude and purified enzyme were sprayed in the fermentation stage of tea. Molecular weight, optimum pH and optimum temperature of enzyme were 12KDa, 8 and 40°C, respectively. Results showed the effect of crude enzyme preparation on qualitative characteristics of tea is more than purified enzyme.
Keywords: Pectin lyase enzyme, Aspergillus niger, Three phase partitioning, Black tea
